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Cells and electropulsation microchambers modeling for linear and nonlinear optical microspectroscopy

TitleCells and electropulsation microchambers modeling for linear and nonlinear optical microspectroscopy
Publication TypePresentazione a Congresso
Year of Publication2018
AuthorsMerla, Caterina, Liberti M., Apollonio F., and Mir L.M.
Conference NameIEEE MTT-S International Microwave Symposium Digest
KeywordsCell membrane permeabilization, Cells, cytology, dosimetry, Electric field distributions, Electric fields, Electric pulse, Electro-pulsation, Electromagnetic emissions, Micro-dosimetry, Molecules, Numerical characterization, Phospholipids, Transmission measurements
Abstract

Mechanisms underlining the cell membrane permeabilization and consequent cellular and intracellular effects are still debated. To shed light on the involvement of phospholipids and water molecules rearrangements in this phenomenon, linear and non-linear optical imaging and microspectroscopy seem particularly appropriate. To combine optical detection with electric pulses used for permeabilization of cells, three delivery setups (microchambers) are presented in this paper. Design and numerical characterization are described and systems performances compared in terms of electromagnetic emissions. The dosimetry of the less radiating microchamber was analyzed in time domain and compared with transmission measurements. Microdosimetry in time domain was also performed to study electric field distribution at cell level. These data give interesting information for interpreting cell microspectroscopic results. © 2018 IEEE.

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85053038269&doi=10.1109%2fMWSYM.2018.8439545&partnerID=40&md5=8686b30923403931491fbf3f31ba6b8b
DOI10.1109/MWSYM.2018.8439545
Citation KeyMerla2018899